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| Registros recuperados: 20 | |
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| Alegbeleye,Oluwadara Oluwaseun; Opeolu,Beatrice Olutoyin; Jackson,Vanessa. |
| Abstract This study was conducted to investigate the occurrence of PAH degrading microorganisms in two river systems in the Western Cape, South Africa and their ability to degrade two PAH compounds: acenaphthene and fluorene. A total of 19 bacterial isolates were obtained from the Diep and Plankenburg rivers among which four were identified as acenaphthene and fluorene degrading isolates. In simulated batch scale experiments, the optimum temperature for efficient degradation of both compounds was determined in a shaking incubator after 14 days, testing at 25 °C, 30 °C, 35 °C, 37 °C, 38 °C, 40 °C and 45 °C followed by experiments in a Stirred Tank Bioreactor using optimum temperature profiles from the batch experiment results. All experiments were run... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Polycyclic aromatic hydrocarbons (PAHs); Bioremediation; Bioreactor; PAH degrading microorganisms. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000200314 |
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| Tagliari,Cristiane Vanessa; Sanson,Raquel K.; Zanette,André; Franco,Telma Teixeira; Soccol,Carlos Ricardo. |
| Various microorganisms including bacteria, yeast and fungi can degrade caffeine. There are few publications about caffeine degradation pathway in filamentous fungi, mainly by solid-state fermentation (SSF). Studies were carried out on degradation of caffeine and their metabolites by filamentous fungi in SSF using coffee husk as substrate. The purpose of this work was to investigate the caffeine degradation pathway by Rhizopus delemar in packed bed column fermenter and to compare this degradation metabolism with glass flasks fermentation. The methylxanthines were quantified by HPLC analysis. The experiments were realized with the optimized conditions in previous experiments: pH 6.5, 28ºC, inoculation rate 10(6) spores/g substrate, aeration rate 60 mL/min... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Decaffeination; Fermentation; Caffeine; Theophylline; Bioreactor; Filamentous fungi. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500035 |
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| Moreira,André Luís; Silva,Adriano Bortolotti da; Santos,Aline; Reis,Caroline Oliveira dos; Landgraf,Paulo Roberto Correa. |
| The aim of the present research was to verify the in vitro growth of orchids in different systems of micropropagation, being cultivated in a bioreactor, with natural ventilation and conventional systems. Cattleya walkeriana plants were obtained from the germination of seeds in culture medium. After 8 months, seedlings with 1 cm of length were placed in a culture vessel according to the treatments, which counted with two micropropagation systems (conventional and natural ventilation) in three media of culture (liquid, solid with 5 or 6g L-1 of agar). Two additional treatments in bioreactor of temporary and continuous immersion were performed. The design was entirely randomized (ERD), consisting of a 2x3 factorial with two additional treatments, totaling 8... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bioreactor; Temporary immersion; Liquid medium; Natural ventilation; In vitro culture. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782013001000012 |
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| Postec, Anne; Lesongeur, Francoise; Pignet, Patricia; Ollivier, B; Querellou, Joel; Godfroy, Anne. |
| The prokaryotic diversity of culturable thermophilic communities of deep-sea hydrothermal chimneys was analysed using a continuous enrichment culture performed in a gas-lift bioreactor, and compared to classical batch enrichment cultures in vials. Cultures were conducted at 60 degrees C and pH 6.5 using a complex medium containing carbohydrates, peptides and sulphur, and inoculated with a sample of a hydrothermal black chimney collected at the Rainbow field, Mid-Atlantic Ridge, at 2,275 m depth. To assess the relevance of both culture methods, bacterial and archaeal diversity was studied using cloning and sequencing, DGGE, and whole-cell hybridisation of 16S rRNA genes. Sequences of heterotrophic microorganisms belonging to the genera Marinitoga,... |
| Tipo: Text |
Palavras-chave: Metabolic interactions; 16S rRNA gene; Thermophiles; Bioreactor; Continuous enrichment cultures; Deep sea hydrothermal vent; Microbial diversity. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-3885.pdf |
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| Leick,Sheilla A.; Oliveira,João G B. De; Tavares,Karoline A.; Lopes,Deize D.; Barana,Ana C.. |
| ABSTRACT This study aimed to evaluate a structured bed reactor with intermittent aeration in the simultaneous removal of Chemical Oxygen Demand (COD) and Total Nitrogen (TN) from sanitary sewage. The reactor was operated for 339 days with a Hydraulic Retention Time (HRT) of 12 hours, temperature of 30 ± 1°C and continuous feeding. Nine trials were carried out in which three different effluent recirculation flow (Qr), 3, 2 and 1 time the incoming flow, were evaluated in three different aeration times, of 1h, 2h, and 3h, in 3 hour cycles. The results showed that different recirculation flows and aeration times did not influence the removal of COD, which reached 89%±12. It was observed that the efficiency of TN removal was higher in the tests with higher... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bioreactor; Wastewater; Denitrification; Nitrification; SND (Simultaneous Nitrification and Denitrification). |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-69162017000601236 |
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| Byrne, Nathalie; Lesongeur, Francoise; Bienvenu, Nadege; Geslin, Claire; Alain, Karine; Prieur, Daniel; Godfroy, Anne. |
| Both cultivation and molecular techniques were used to investigate the microbial diversity and dynamic of a deep-sea vent chimney. The enrichment cultures performed in a gas-lift bioreactor were inoculated with a black smoker chimney sample collected on TAG site on the mid-Atlantic ridge. To mimic as close as possible environmental conditions, the cultures were performed in oligotrophic medium with nitrogen, hydrogen and carbon dioxide (N-2/H-2/CO2) gas sweeping. Also, the temperature was first settled at a temperature of 85A degrees C and colloidal sulphur was added. Then, the temperature was lowered to 60A degrees C and sulphur was omitted. Archaeal and bacterial diversity was studied in both culture and natural samples. Through 16S rRNA gene sequences... |
| Tipo: Text |
Palavras-chave: 16S rRNA genes; Oligotrophic condition; Environmental condition; Bioreactor; Continuous enrichment culture; Microbial diversity; Deep sea hydrothermal vent. |
| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/2009/publication-6920.pdf |
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| Habibi,Peyman; Piri,Khosro; Deljo,Ali; Moghadam,Yaser Ahmadi; Ghiasvand,Taiebeh. |
| The aim of this study was to use the, hairy root system for increasing the scopolamine content in Atropa belladonna. Agrobacterium rhizogenes ATCC15834 was utilized to produce hairy roots. The culture was carried out in a 1.5-l bioreactor using the inoculum size of 0.5 g fr. wt of 10-day-old hairy roots and various parameters, including agitation, aeration, conductivity and the consumption of sucrose were evaluated. Results revealed that the highest amount of scopolamine production (1.59 mg/g-1 dry wt) occurred in the bioreactor with aeration and agitation 1.25 vvm (volume per volume per minute) and 70 rpm, respectively. Study of conductivity and the consumption of sucrose showed that the highest amount of sucrose consumption and the highest amount of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Atropa belladonna; Hairy root; Scopolamine; Bioreactor. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000200166 |
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| Postec, Anne; Pignet, Patricia; Cueff-gauchard, Valerie; Schmitt, Anne; Querellou, Joel; Godfroy, Anne. |
| The hyperthermophilic archaeon Thermococcus hydrothermalis was cultivated in continuous culture in a gas-lift bioreactor in the absence of elemental sulphur on both proteinaceous and maltose-containing media. Optimal conditions (pH, temperature and gas flow rate), determined on complex media that yielded maximal growth rate and maximal steady state cell density, were obtained at 80degreesC, pH 6 and gas sparging at 0.2 v v(-1) min(-1). Higher steady state cell densities were obtained on a medium containing maltose and yeast extract. In order to design a defined and minimal media, the nutritional requirements of T hydrothermalis were then investigated using continuous culture in the absence of elemental sulphur in the gas-lift bioreactor. First, the complex... |
| Tipo: Text |
Palavras-chave: Bioreactor; Defined and minimal media; Culture optimisation; Gas lift bioreactor; Thermococcus hydrothermalis. |
| Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/2005/publication-365.pdf |
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| Brillet-viel, Anne; Pilet, Marie-france; Courcoux, Philippe; Prevost, Hervé; Leroi, Francoise. |
| Optimization of Carnobacterium divergens V41 growth and bacteriocin activity in a culture medium deprived of animal protein, needs for food bioprotection, was performed by using a statistical approach. In a screening experiment, twelve factors (pH, temperature, carbohydrates, NaCl, yeast extract, soy peptone, sodium acetate, ammonium citrate, magnesium sulphate, manganese sulphate, ascorbic acid and thiamine) were tested for their influence on the maximal growth and bacteriocin activity using a two-level incomplete factorial design with 192 experiments performed in microtiter plate wells. Based on results, a basic medium was developed and three variables (pH, temperature and carbohydrates concentration) were selected for a scale-up study in bioreactor. A... |
| Tipo: Text |
Palavras-chave: Optimization; Carnobacterium; Bacteriocin; Experimental design; Bioreactor; Growth; Cold-smoked salmon. |
| Ano: 2016 |
URL: https://archimer.ifremer.fr/doc/00345/45615/45236.pdf |
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| Fang,Qiangyi; Shen,Bingqian. |
| Response surface methodology was undertaken to optimize the polyethylenimine-mediated transient transfection of suspension cultured HEK 293-F cells. A total of 15 combinations were designed according to Box-Behnken design to identify the effects of DNA concentration, polyethylenimine concentration and incubation time on transient transfection efficiency. The highest integral optic density of green fluorescent protein presenting r-protein yield was accessed using a DNA concentration of 1.75 µg/mL, a polyethylenimine concentration of 10.5 µg/mL, and an incubation time of 11.8 min. Analysis of variance demonstrated that the experimental values fit well with a quadratic model. The RSM-optimized transient transfection resulted in greater production of human... |
| Tipo: Journal article |
Palavras-chave: Bioreactor; Human tissue kallikrein; Transient gene expression. |
| Ano: 2010 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000500010 |
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| Borges,Ana Kleiber Pessoa; Tauk-Tornisielo,Sâmia Maria; Domingos,Roberto Naves; Angelis,Dejanira de Franceschi de. |
| The aim of this work was to study the constructed wetland system for the treatment of water from the Corumbataí river simulated on a laboratory scale. The parameters analyzed at different points of the system were ammonia, biochemical demand for oxygen (BDO), chemical demand for oxygen (CDO), chlorides, apparent color, conductivity, dissolved oxygen, magnesium (Mg), sodium (Na), potassium (K), silicon (Si), total phosphorous, total coliforms and Escherichia coli, total dissolved solids (TDS), turbidity, and macrophyte biomass. The results demonstrated that this alternative water treatment system was effective in removing the microorganisms (total coliforms and E. coli), among other parameters analyzed, for varying periods of the treatment, promoting... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Aquatic macrophytes; Filtering soil; Microfiltration; Bioreactor; Microorganisms. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000600024 |
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| Gamal,Rawia F.; Abdelhady,Hemmat M.; Khodair,Taha A.; El-Tayeb,Tarek S.; Hassan,Enas A.; Aboutaleb,Khadiga A.. |
| The present study aimed at developing a strategy to improve the volumetric production of PHAs by Pseudomonas fluorescens S48 using waste frying oil (WFO) as the sole carbon source. For this purpose, several cultivations were set up to steadily improve nutrients supply to attain high cell density and high biopolymer productivity. The production of PHAs was examined in a 14 L bioreactor as one-stage batch, two-stage batch, and high-cell-density fed-batch cultures. The highest value of polymer content in one-stage bioreactor was obtained after 60 h (33.7%). Whereas, the two-stage batch culture increased the polymer content to 50.1% after 54 h. High-cell-density (0.64 g/L) at continuous feeding rate 0.55 mL/l/h of WFO recorded the highest polymer content after... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Pseudomonas fluorescens S48; Biopolymer; PHAs; Batch; Two-stage batch; High-celldensity fed batch; Bioreactor; Recovery. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000200034 |
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| Registros recuperados: 20 | |
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